105 bacteria per well Search Results


staphylococcus aureus atcc 25923 bacterial culture  (ATCC)
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ATCC staphylococcus aureus atcc 25923 bacterial culture
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bacillus subtilis group bacillus subtilis atcc  (ATCC)
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ATCC bacillus subtilis group bacillus subtilis atcc
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lexsy neo antibiotic  (Jena Bioscience)
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Jena Bioscience lexsy neo antibiotic
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human igg fc fragments  (Bethyl)
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virus strains  (ATCC)
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ATCC virus strains
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24-well microtiter plates greiner bio-one  (Greiner Bio)
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cd11b antibody  (Miltenyi Biotec)
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Miltenyi Biotec cd11b antibody
Influence of HLA-A11/DR1 on macrophage mitochondrial ROS-associated bacterial clearance. BMDM cells from WT and HLA-A11/DR1 mice were subjected to infection with S. suis at a MOI of 10. Subsequently, gentamicin treatment was applied 60 minutes post-infection to eliminate extracellular bacteria. The quantification of live bacteria was accomplished by plating on THY agar supplemented with ampicillin at (A) 1 h or (B) 6 h post-infection. Mitochondrial ROS production was assessed under the following conditions: (C) BMDM cells exposed to S. suis at MOI 20 at the specified time points in vitro , and (D) <t>CD11b</t> + cells collected from WT and HLA-A11/DR1 mice during S. suis infection. Data are represented as mean ± SEM. * P < 0.05; ** P < 0.01.
Cd11b Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacteria  (ATCC)
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ATCC bacteria
Influence of HLA-A11/DR1 on macrophage mitochondrial ROS-associated bacterial clearance. BMDM cells from WT and HLA-A11/DR1 mice were subjected to infection with S. suis at a MOI of 10. Subsequently, gentamicin treatment was applied 60 minutes post-infection to eliminate extracellular bacteria. The quantification of live bacteria was accomplished by plating on THY agar supplemented with ampicillin at (A) 1 h or (B) 6 h post-infection. Mitochondrial ROS production was assessed under the following conditions: (C) BMDM cells exposed to S. suis at MOI 20 at the specified time points in vitro , and (D) <t>CD11b</t> + cells collected from WT and HLA-A11/DR1 mice during S. suis infection. Data are represented as mean ± SEM. * P < 0.05; ** P < 0.01.
Bacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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105 bacteria  (ATCC)
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ATCC 105 bacteria
Influence of HLA-A11/DR1 on macrophage mitochondrial ROS-associated bacterial clearance. BMDM cells from WT and HLA-A11/DR1 mice were subjected to infection with S. suis at a MOI of 10. Subsequently, gentamicin treatment was applied 60 minutes post-infection to eliminate extracellular bacteria. The quantification of live bacteria was accomplished by plating on THY agar supplemented with ampicillin at (A) 1 h or (B) 6 h post-infection. Mitochondrial ROS production was assessed under the following conditions: (C) BMDM cells exposed to S. suis at MOI 20 at the specified time points in vitro , and (D) <t>CD11b</t> + cells collected from WT and HLA-A11/DR1 mice during S. suis infection. Data are represented as mean ± SEM. * P < 0.05; ** P < 0.01.
105 Bacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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opa buffer  (Thermo Fisher)
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Thermo Fisher opa buffer
Influence of HLA-A11/DR1 on macrophage mitochondrial ROS-associated bacterial clearance. BMDM cells from WT and HLA-A11/DR1 mice were subjected to infection with S. suis at a MOI of 10. Subsequently, gentamicin treatment was applied 60 minutes post-infection to eliminate extracellular bacteria. The quantification of live bacteria was accomplished by plating on THY agar supplemented with ampicillin at (A) 1 h or (B) 6 h post-infection. Mitochondrial ROS production was assessed under the following conditions: (C) BMDM cells exposed to S. suis at MOI 20 at the specified time points in vitro , and (D) <t>CD11b</t> + cells collected from WT and HLA-A11/DR1 mice during S. suis infection. Data are represented as mean ± SEM. * P < 0.05; ** P < 0.01.
Opa Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 mg/ml dispase  (Thermo Fisher)
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Thermo Fisher 1 mg/ml dispase
Influence of HLA-A11/DR1 on macrophage mitochondrial ROS-associated bacterial clearance. BMDM cells from WT and HLA-A11/DR1 mice were subjected to infection with S. suis at a MOI of 10. Subsequently, gentamicin treatment was applied 60 minutes post-infection to eliminate extracellular bacteria. The quantification of live bacteria was accomplished by plating on THY agar supplemented with ampicillin at (A) 1 h or (B) 6 h post-infection. Mitochondrial ROS production was assessed under the following conditions: (C) BMDM cells exposed to S. suis at MOI 20 at the specified time points in vitro , and (D) <t>CD11b</t> + cells collected from WT and HLA-A11/DR1 mice during S. suis infection. Data are represented as mean ± SEM. * P < 0.05; ** P < 0.01.
1 Mg/Ml Dispase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l. brevis cd2  (VSL Pharmaceuticals)
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VSL Pharmaceuticals l. brevis cd2
Influence of HLA-A11/DR1 on macrophage mitochondrial ROS-associated bacterial clearance. BMDM cells from WT and HLA-A11/DR1 mice were subjected to infection with S. suis at a MOI of 10. Subsequently, gentamicin treatment was applied 60 minutes post-infection to eliminate extracellular bacteria. The quantification of live bacteria was accomplished by plating on THY agar supplemented with ampicillin at (A) 1 h or (B) 6 h post-infection. Mitochondrial ROS production was assessed under the following conditions: (C) BMDM cells exposed to S. suis at MOI 20 at the specified time points in vitro , and (D) <t>CD11b</t> + cells collected from WT and HLA-A11/DR1 mice during S. suis infection. Data are represented as mean ± SEM. * P < 0.05; ** P < 0.01.
L. Brevis Cd2, supplied by VSL Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Influence of HLA-A11/DR1 on macrophage mitochondrial ROS-associated bacterial clearance. BMDM cells from WT and HLA-A11/DR1 mice were subjected to infection with S. suis at a MOI of 10. Subsequently, gentamicin treatment was applied 60 minutes post-infection to eliminate extracellular bacteria. The quantification of live bacteria was accomplished by plating on THY agar supplemented with ampicillin at (A) 1 h or (B) 6 h post-infection. Mitochondrial ROS production was assessed under the following conditions: (C) BMDM cells exposed to S. suis at MOI 20 at the specified time points in vitro , and (D) CD11b + cells collected from WT and HLA-A11/DR1 mice during S. suis infection. Data are represented as mean ± SEM. * P < 0.05; ** P < 0.01.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Human HLA prolongs the host inflammatory response in Streptococcus suis serotype 2 infection compared to mouse H2 molecules

doi: 10.3389/fcimb.2023.1285055

Figure Lengend Snippet: Influence of HLA-A11/DR1 on macrophage mitochondrial ROS-associated bacterial clearance. BMDM cells from WT and HLA-A11/DR1 mice were subjected to infection with S. suis at a MOI of 10. Subsequently, gentamicin treatment was applied 60 minutes post-infection to eliminate extracellular bacteria. The quantification of live bacteria was accomplished by plating on THY agar supplemented with ampicillin at (A) 1 h or (B) 6 h post-infection. Mitochondrial ROS production was assessed under the following conditions: (C) BMDM cells exposed to S. suis at MOI 20 at the specified time points in vitro , and (D) CD11b + cells collected from WT and HLA-A11/DR1 mice during S. suis infection. Data are represented as mean ± SEM. * P < 0.05; ** P < 0.01.

Article Snippet: To selectively characterize monocytes/macrophages, an acutely isolated monocytes/macrophages fraction was obtained using MACS microbeads (Miltenyi Biotec) conjugated with CD11b antibody.

Techniques: Infection, Bacteria, In Vitro